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I'm trying to detect mutation using restriction site RFLP.
I want to survey the sequence for a plasmid and detect sequences which are not normally restriction sites, but would become restriction sites if a G residue in the sequence was converted to an A, T or a C.
I imagine there is some straight PERL or Python script that can be made to do this, I just don't have the time or the expertise.
Please quote price and time frame for the job.